Abstract
SNAP-25 is a neuronal SNARE protein required for synaptic vesicle exocytosis and neurite outgrowth. Here we show that in addition to synaptic staining, SNAP-25 immunoreactivity is also localized to an intracellular, perinuclear compartment of retinal neurons. Double-labeling with an antibody against the 58 kD resident protein of the trans-golgi network indicates that the intracellular SNAP-25 is localized to the Golgi complex. Immuno-electron microscopic localization of SNAP-25 confirmed its presence on the Golgi apparatus of photoreceptors, bipolar cells, amacrine cells and ganglion cells in the retina. These data implicate SNAP-25 in the trafficking of Golgi- derived vesicles in neurons in addition to the synaptic vesicle cycle.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 85-88 |
| Number of pages | 4 |
| Journal | NeuroReport |
| Volume | 11 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 17 2000 |
| Externally published | Yes |
Keywords
- Golgi
- Neurons
- Rat
- Retina
- SNAP-25
ASJC Scopus subject areas
- General Neuroscience