Spectral Doppler optical coherence tomography imaging of localized ischemic stroke in a mouse model

Lingfeng Yu, Elaine Nguyen, Gangjun Liu, Bernard Choi, Zhongping Chen

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


We report the use of spectral Doppler optical coherence tomography imaging (SDOCTI) for quantitative evaluation of dynamic blood circulation before and after a localized ischemic stroke in a mouse model. Rose Bengal photodynamic therapy (PDT) is used as a noninvasive means for inducing localized ischemia in cortical microvasculature of the mouse. Fast, repeated Doppler optical coherence tomography scans across vessels of interest are performed to record flow dynamic information with high temporal resolution. Doppler-angle-independent flow indices are used to quantify vascular conditions before and after the induced ischemia by the photocoagulation of PDT. The higher (or lower) flow resistive indices are associated with higher (or lower) resistance states that are confirmed by laser speckle flow index maps (of laser speckle imaging). Our in vivo experiments shows that SDOCTI can provide complementary quantified flow information that is an alternative to blood volume measurement, and can be used as a means for cortical microvasculature imaging well suited for small animal studies.

Original languageEnglish (US)
Article number066006
JournalJournal of biomedical optics
Issue number6
StatePublished - Nov 2010
Externally publishedYes


  • Doppler
  • biomedical optics
  • laser Doppler velocimetry
  • laser speckle imaging
  • tomography

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Biomedical Engineering


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