Substance P opens cation channels and closes potassium channels in rat locus coeruleus neurons

K. Z. Shen; R. A. North

doi:10.1016/0306-4522(92)90428-5

Substance P opens cation channels and closes potassium channels in rat locus coeruleus neurons

K. Z. Shen, R. A. North

Neurology

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113 Scopus citations

Abstract

Whole-cell recordings were made from neurons of the rat locus coeruleus in a tissue slice removed from rat brain. Substance P caused an inward current in cells voltage-clamped at -60 mV. The effect of substance P was concentration-dependent (30nM-3 μM) and was mimicked by similar concentrations of substance K and neuromedin K. The inward current resulted predominately from an increase in membrane cation conductance; in potassium-free solutions it reversed polarity at about 12 mV. Substance P also reduced the conductance of an inwardly rectifying potassium current; this action was studied with low external sodium concentration. p It is concluded that substance P excites rat locus coeruleus neurons by activating an intracellular transduction pathway leading to both cation conductance increase and potassium conductance decrease.

Original language	English (US)
Pages (from-to)	345-353
Number of pages	9
Journal	Neuroscience
Volume	50
Issue number	2
DOIs	https://doi.org/10.1016/0306-4522(92)90428-5
State	Published - Sep 1992

ASJC Scopus subject areas

General Neuroscience

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title = "Substance P opens cation channels and closes potassium channels in rat locus coeruleus neurons",

abstract = "Whole-cell recordings were made from neurons of the rat locus coeruleus in a tissue slice removed from rat brain. Substance P caused an inward current in cells voltage-clamped at -60 mV. The effect of substance P was concentration-dependent (30nM-3 μM) and was mimicked by similar concentrations of substance K and neuromedin K. The inward current resulted predominately from an increase in membrane cation conductance; in potassium-free solutions it reversed polarity at about 12 mV. Substance P also reduced the conductance of an inwardly rectifying potassium current; this action was studied with low external sodium concentration. p It is concluded that substance P excites rat locus coeruleus neurons by activating an intracellular transduction pathway leading to both cation conductance increase and potassium conductance decrease.",

author = "Shen, {K. Z.} and North, {R. A.}",

note = "Funding Information: Acknowledgement--This work was supported by grants from the U.S. Department of Health and Human Services.",

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T1 - Substance P opens cation channels and closes potassium channels in rat locus coeruleus neurons

AU - Shen, K. Z.

AU - North, R. A.

N1 - Funding Information: Acknowledgement--This work was supported by grants from the U.S. Department of Health and Human Services.

PY - 1992/9

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N2 - Whole-cell recordings were made from neurons of the rat locus coeruleus in a tissue slice removed from rat brain. Substance P caused an inward current in cells voltage-clamped at -60 mV. The effect of substance P was concentration-dependent (30nM-3 μM) and was mimicked by similar concentrations of substance K and neuromedin K. The inward current resulted predominately from an increase in membrane cation conductance; in potassium-free solutions it reversed polarity at about 12 mV. Substance P also reduced the conductance of an inwardly rectifying potassium current; this action was studied with low external sodium concentration. p It is concluded that substance P excites rat locus coeruleus neurons by activating an intracellular transduction pathway leading to both cation conductance increase and potassium conductance decrease.

AB - Whole-cell recordings were made from neurons of the rat locus coeruleus in a tissue slice removed from rat brain. Substance P caused an inward current in cells voltage-clamped at -60 mV. The effect of substance P was concentration-dependent (30nM-3 μM) and was mimicked by similar concentrations of substance K and neuromedin K. The inward current resulted predominately from an increase in membrane cation conductance; in potassium-free solutions it reversed polarity at about 12 mV. Substance P also reduced the conductance of an inwardly rectifying potassium current; this action was studied with low external sodium concentration. p It is concluded that substance P excites rat locus coeruleus neurons by activating an intracellular transduction pathway leading to both cation conductance increase and potassium conductance decrease.

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Substance P opens cation channels and closes potassium channels in rat locus coeruleus neurons

Abstract

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