Surface-enhanced resonance raman scattering spectroscopy applied to phytochrome and its model compounds. 2. Phytochrome and phycocyanin chromophorest

David L. Farrens; Randall E. Holt; Bernard N. Rospendowski; Pill Soon Song; Therese M. Cotton

doi:10.1021/ja00208a004

Surface-enhanced resonance raman scattering spectroscopy applied to phytochrome and its model compounds. 2. Phytochrome and phycocyanin chromophorest

David L. Farrens, Randall E. Holt, Bernard N. Rospendowski, Pill Soon Song, Therese M. Cotton

Research output: Contribution to journal › Article › peer-review

60 Scopus citations

Abstract

Surface-enhanced resonance Raman scattering (SERRS) spectra of phytochrome at 77 K are reported. The spectra reveal significant differences between Pr and Pfr forms of phytochrome. SERRS spectra of C-phycocyanin Z, Z, Z- and Z, Z, E-chromopeptide isomers at 77 K are also reported. The phycocyanin chromopeptide studies are used to provide a basis for interpreting the phytochrome SERRS spectra. The spectra indicate that photoisomerization of chromophores from C-phycocyanin chromopeptides (from a Z, Z, Z to a Z, Z, E configuration) is detectable with SERRS. Comparison of SERRS spectra between the Pr and Pfr forms of 124-kDa phytochrome adsorbed on silver colloids demonstrates that the chromophore undergoes a Z →E isomerization during the Pr →Pfr phototransformation. However, the overall chromophore conformations are likely to be conserved for the native Pr and Pfr phytochrome species.

Original language	English (US)
Pages (from-to)	9162-9169
Number of pages	8
Journal	Journal of the American Chemical Society
Volume	111
Issue number	26
DOIs	https://doi.org/10.1021/ja00208a004
State	Published - Dec 1989
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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Surface-enhanced resonance raman scattering spectroscopy applied to phytochrome and its model compounds. 2. Phytochrome and phycocyanin chromophorest. / Farrens, David L.; Holt, Randall E.; Rospendowski, Bernard N. et al.
In: Journal of the American Chemical Society, Vol. 111, No. 26, 12.1989, p. 9162-9169.

Research output: Contribution to journal › Article › peer-review

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title = "Surface-enhanced resonance raman scattering spectroscopy applied to phytochrome and its model compounds. 2. Phytochrome and phycocyanin chromophorest",

abstract = "Surface-enhanced resonance Raman scattering (SERRS) spectra of phytochrome at 77 K are reported. The spectra reveal significant differences between Pr and Pfr forms of phytochrome. SERRS spectra of C-phycocyanin Z, Z, Z- and Z, Z, E-chromopeptide isomers at 77 K are also reported. The phycocyanin chromopeptide studies are used to provide a basis for interpreting the phytochrome SERRS spectra. The spectra indicate that photoisomerization of chromophores from C-phycocyanin chromopeptides (from a Z, Z, Z to a Z, Z, E configuration) is detectable with SERRS. Comparison of SERRS spectra between the Pr and Pfr forms of 124-kDa phytochrome adsorbed on silver colloids demonstrates that the chromophore undergoes a Z →E isomerization during the Pr →Pfr phototransformation. However, the overall chromophore conformations are likely to be conserved for the native Pr and Pfr phytochrome species.",

author = "Farrens, {David L.} and Holt, {Randall E.} and Rospendowski, {Bernard N.} and Song, {Pill Soon} and Cotton, {Therese M.}",

year = "1989",

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T1 - Surface-enhanced resonance raman scattering spectroscopy applied to phytochrome and its model compounds. 2. Phytochrome and phycocyanin chromophorest

AU - Farrens, David L.

AU - Holt, Randall E.

AU - Rospendowski, Bernard N.

AU - Song, Pill Soon

AU - Cotton, Therese M.

PY - 1989/12

Y1 - 1989/12

N2 - Surface-enhanced resonance Raman scattering (SERRS) spectra of phytochrome at 77 K are reported. The spectra reveal significant differences between Pr and Pfr forms of phytochrome. SERRS spectra of C-phycocyanin Z, Z, Z- and Z, Z, E-chromopeptide isomers at 77 K are also reported. The phycocyanin chromopeptide studies are used to provide a basis for interpreting the phytochrome SERRS spectra. The spectra indicate that photoisomerization of chromophores from C-phycocyanin chromopeptides (from a Z, Z, Z to a Z, Z, E configuration) is detectable with SERRS. Comparison of SERRS spectra between the Pr and Pfr forms of 124-kDa phytochrome adsorbed on silver colloids demonstrates that the chromophore undergoes a Z →E isomerization during the Pr →Pfr phototransformation. However, the overall chromophore conformations are likely to be conserved for the native Pr and Pfr phytochrome species.

AB - Surface-enhanced resonance Raman scattering (SERRS) spectra of phytochrome at 77 K are reported. The spectra reveal significant differences between Pr and Pfr forms of phytochrome. SERRS spectra of C-phycocyanin Z, Z, Z- and Z, Z, E-chromopeptide isomers at 77 K are also reported. The phycocyanin chromopeptide studies are used to provide a basis for interpreting the phytochrome SERRS spectra. The spectra indicate that photoisomerization of chromophores from C-phycocyanin chromopeptides (from a Z, Z, Z to a Z, Z, E configuration) is detectable with SERRS. Comparison of SERRS spectra between the Pr and Pfr forms of 124-kDa phytochrome adsorbed on silver colloids demonstrates that the chromophore undergoes a Z →E isomerization during the Pr →Pfr phototransformation. However, the overall chromophore conformations are likely to be conserved for the native Pr and Pfr phytochrome species.

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Surface-enhanced resonance raman scattering spectroscopy applied to phytochrome and its model compounds. 2. Phytochrome and phycocyanin chromophorest

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