@article{eaad67e6e7354558abde7f211ddc476a,
title = "Synthesis of a far-red fluorophore and its use as an esterase probe in living cells",
abstract = "We report the synthesis of a new far-red fluorophore, 1,3-dichloro-7-hydroxy-2H-spiro[acridine-9,1′-cyclohexane]-2′,5′-diene-2,4′-dione (DSACO), which was modified to make two esterase probes: DSACO-2-AME and DSACO-7-AME. Both probes act as {"}turn-on{"} substrates for esterases and lipases. DSACO-2-AME exhibited efficient esterase-activated fluorescence inside living cells and is a stable, far-red alternative for the widely-used fluorescein diacetate.",
author = "Levine, {Samantha R.} and Beatty, {Kimberly E.}",
note = "Funding Information: We thank Dr. Hannah Zane for her help with tissue culture during this project. Ms. Katie Tallman and Dr. Jonathan White provided helpful critiques of this manuscript. We thank Dr. Stefanie Kaech Petrie of OHSU{\textquoteright}s Advanced Light Microscopy Core for her help with imaging. HRMS data were collected on Portland State University{\textquoteright}s ThermoElectron LTQ-Orbitrap Discovery (NSF instrument grant 0741993) with the help of Dr. Andrea DeBarber. K. E. B. is grateful for generous funding from the OHSU Center for Spatial Systems Biomedicine, the Knight Cancer Institute, and the Medical Research Foundation of Oregon. S. R. L. was partially supported by a training grant (T32-AI07472) entitled {"}Interactions at the Microbe/Host Interface{"}. Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2016.",
year = "2016",
month = jan,
day = "31",
doi = "10.1039/c5cc08764c",
language = "English (US)",
volume = "52",
pages = "1835--1838",
journal = "Chemical Communications",
issn = "1359-7345",
publisher = "Royal Society of Chemistry",
number = "9",
}