Synthetic phosphopeptide immunogens yield activation-specific antibodies to the c-erbB-2 receptor

Richard J. Epstein, Brian J. Druker, Thomas M. Roberts, Charles D. Stiles

Research output: Contribution to journalArticlepeer-review

58 Scopus citations


We inoculated rabbits with synthetic phosphopeptides, duplicating a major autophosphorylation site of the c-erbB-2 protooncogene product. The rabbits produced antisera that, after reverse immunoaffinity purification, selectively recognize the erbB-2 protein in its enzymatically active configuration. These anti-phosphopeptide antisera identify a subset of erbB-2-positive human cell lines wherein the protein is constitutively active as a tyrosine kinase. Synthetic phosphopeptides incorporating informative protein phosphorylation sites may prove useful for generating antibodies that indicate the activation state of additional tyrosine kinases and perhaps other proteins phosphorylated on serine and threonine residues.

Original languageEnglish (US)
Pages (from-to)10435-10439
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number21
StatePublished - 1992
Externally publishedYes


  • Breast neoplasms
  • Oncogenes
  • Phosphorylation
  • Tyrosine kinases

ASJC Scopus subject areas

  • General


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