The effect of photodynamic action on corneal cells in tissue culture

Moshe Lahav; Daniel M. Albert; Nancy L. Robinson; David K. Dueker

doi:10.1016/0014-4835(75)90224-9

The effect of photodynamic action on corneal cells in tissue culture

Moshe Lahav, Daniel M. Albert, Nancy L. Robinson, David K. Dueker

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The effect of photodynamic inactivation on corneal epithelial, stromal and endothelial cells was investigated in tissue cultures. In contrast to reports regarding in vivo studies, the in vitro treatment caused significant degeneration and growth inhibition of stromal corneal cells and less marked changes of epithelial corneal cells in tissue cultures. Stationary endothelial cells appeared least effected. The neutral red was seen in the intracytoplasmic granules and in time localized mostly in the perinuclear area, and to a lesser extent within the nucleus. The in vitro study of actively growing corneal cells appears to be a sensitive indicator of cell damage following photoinactivation.

Original language	English (US)
Pages (from-to)	571-583
Number of pages	13
Journal	Experimental Eye Research
Volume	20
Issue number	6
DOIs	https://doi.org/10.1016/0014-4835(75)90224-9
State	Published - Jun 1975
Externally published	Yes

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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title = "The effect of photodynamic action on corneal cells in tissue culture",

abstract = "The effect of photodynamic inactivation on corneal epithelial, stromal and endothelial cells was investigated in tissue cultures. In contrast to reports regarding in vivo studies, the in vitro treatment caused significant degeneration and growth inhibition of stromal corneal cells and less marked changes of epithelial corneal cells in tissue cultures. Stationary endothelial cells appeared least effected. The neutral red was seen in the intracytoplasmic granules and in time localized mostly in the perinuclear area, and to a lesser extent within the nucleus. The in vitro study of actively growing corneal cells appears to be a sensitive indicator of cell damage following photoinactivation.",

author = "Moshe Lahav and Albert, {Daniel M.} and Robinson, {Nancy L.} and Dueker, {David K.}",

year = "1975",

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doi = "10.1016/0014-4835(75)90224-9",

language = "English (US)",

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pages = "571--583",

journal = "Experimental Eye Research",

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AU - Lahav, Moshe

AU - Albert, Daniel M.

AU - Robinson, Nancy L.

AU - Dueker, David K.

PY - 1975/6

Y1 - 1975/6

N2 - The effect of photodynamic inactivation on corneal epithelial, stromal and endothelial cells was investigated in tissue cultures. In contrast to reports regarding in vivo studies, the in vitro treatment caused significant degeneration and growth inhibition of stromal corneal cells and less marked changes of epithelial corneal cells in tissue cultures. Stationary endothelial cells appeared least effected. The neutral red was seen in the intracytoplasmic granules and in time localized mostly in the perinuclear area, and to a lesser extent within the nucleus. The in vitro study of actively growing corneal cells appears to be a sensitive indicator of cell damage following photoinactivation.

AB - The effect of photodynamic inactivation on corneal epithelial, stromal and endothelial cells was investigated in tissue cultures. In contrast to reports regarding in vivo studies, the in vitro treatment caused significant degeneration and growth inhibition of stromal corneal cells and less marked changes of epithelial corneal cells in tissue cultures. Stationary endothelial cells appeared least effected. The neutral red was seen in the intracytoplasmic granules and in time localized mostly in the perinuclear area, and to a lesser extent within the nucleus. The in vitro study of actively growing corneal cells appears to be a sensitive indicator of cell damage following photoinactivation.

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JO - Experimental Eye Research

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The effect of photodynamic action on corneal cells in tissue culture

Abstract

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