TY - JOUR
T1 - The effects of photodynamic therapy with blue light and papain-based gel associated with Urucum, on collagen and fibroblasts
T2 - a spectroscopic and cytotoxicity analysis
AU - Silva, Zenildo Santos
AU - França, Cristiane Miranda
AU - Araújo Prates, Renato
AU - Botta, Sergio Brossi
AU - Ferrari, Raquel Agnelli Mesquita
AU - Ana, Patricia Aparecida
AU - Pavani, Christiane
AU - Fernandes, Kristianne Porta Santos
AU - de Fátima Teixeira da Silva, Daniela
AU - Hamblin, Michael R.
AU - Bussadori, Sandra Kalil
N1 - Publisher Copyright:
© 2019, Springer-Verlag London Ltd., part of Springer Nature.
PY - 2020/4/1
Y1 - 2020/4/1
N2 - Papacarie Duo™ is clinically used and has proven effectiveness; however, it is necessary to improve its antimicrobial action. The combined treatment of Papacarie Duo™ with Urucum (Bixa Orellana) could create a potential tool for dental caries treatment; its extract obtained from the seeds’ pericarp contains a water-soluble primary pigment (cis-bixin) with smaller amounts of other carotenoids. The dicarboxylic acid salts of cis-norbixin and trans-norbixin occur in heated alkaline solutions. To analyze the absorption spectra and cytotoxicity (with human dermal fibroblasts) in different concentrations of Urucum, associated or not with Papacarie Duo™, we performed this in vitro study. The effects of pure Urucum, Papacarie Duo™, and PapaUrucum™ on the microstructure of collagen were also analyzed. The application of papain-based gel with Urucum did not present cytotoxicity, its exhibit UV absorption spectrum peak around 460 ± 20 nm. Also, it showed that the compound used did not alter the chemical structure of collagen. Consequently, this product could be used as a chemomechanical method to remove dentin caries as well as being a potential product for antimicrobial photodynamic therapy (aPDT) application.
AB - Papacarie Duo™ is clinically used and has proven effectiveness; however, it is necessary to improve its antimicrobial action. The combined treatment of Papacarie Duo™ with Urucum (Bixa Orellana) could create a potential tool for dental caries treatment; its extract obtained from the seeds’ pericarp contains a water-soluble primary pigment (cis-bixin) with smaller amounts of other carotenoids. The dicarboxylic acid salts of cis-norbixin and trans-norbixin occur in heated alkaline solutions. To analyze the absorption spectra and cytotoxicity (with human dermal fibroblasts) in different concentrations of Urucum, associated or not with Papacarie Duo™, we performed this in vitro study. The effects of pure Urucum, Papacarie Duo™, and PapaUrucum™ on the microstructure of collagen were also analyzed. The application of papain-based gel with Urucum did not present cytotoxicity, its exhibit UV absorption spectrum peak around 460 ± 20 nm. Also, it showed that the compound used did not alter the chemical structure of collagen. Consequently, this product could be used as a chemomechanical method to remove dentin caries as well as being a potential product for antimicrobial photodynamic therapy (aPDT) application.
KW - Cytotoxicity
KW - Dentin
KW - Fourier transform infrared spectroscopy
KW - Papain-based gel
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U2 - 10.1007/s10103-019-02857-7
DO - 10.1007/s10103-019-02857-7
M3 - Letter
C2 - 31407100
AN - SCOPUS:85070785786
SN - 0268-8921
VL - 35
SP - 767
EP - 775
JO - Lasers In Medical Science
JF - Lasers In Medical Science
IS - 3
ER -