TY - JOUR
T1 - The role of interleukin-6 in endotoxin induced uveitis in wild-type and IL-6 deficient mice
AU - Kievit, P.
AU - Park, J. M.
AU - O'Rourke, L. M.
AU - Planck, S. R.
AU - Rosenbaum, J. T.
PY - 1996/2/15
Y1 - 1996/2/15
N2 - Purpose: Interleukin-6 is a cytokine that is present in ocular fluids from inflamed eyes and is proposed to serve as a major mediator in eye inflammation. Injection of recombinant human IL-6 in rats (Hoekzema R et al. 1992. Invest. Ophthal. Vis. Sci. 33; 532-539) induced acute uveitis, suggesting that IL-6 is a key cytokine in the pathogenesis. In this study, the requirement for IL-6 in endotoxin induced uveitis (EIU) was examined using IL-6 deficient mice. In addition, the capability of murine IL-6 to induce uveitis was tested. Methods: Endotoxin (250 ng) was injected bilaterally into the posterior segment of the eyes in IL-6 deficient and wild type mice (n=8). After 24 hours, eyes were processed for histology and inflammation was determined by scoring the number of infiltrating cells in anterior chamber and posterior segment. To test the ability of IL-6 to induce uveitis, various concentrations of murine IL-6 (1, 4, 10, 20, 200 ng per eye; n=4 per dose) were injected in the posterior segment. Results: Histological examination of the IL-6 deficient and wild-type mice showed no significant difference (p=0.07) in the number of infiltrating cells (63.5±16.9 and 26.5±8.81 respectively). Injection of murine IL-6 in the posterior chamber did not cause significant inflammation at any dose tested. Conclusion: These results show that IL-6, although highly expressed in EIU, is not a key cytokine in the induction of inflammation.
AB - Purpose: Interleukin-6 is a cytokine that is present in ocular fluids from inflamed eyes and is proposed to serve as a major mediator in eye inflammation. Injection of recombinant human IL-6 in rats (Hoekzema R et al. 1992. Invest. Ophthal. Vis. Sci. 33; 532-539) induced acute uveitis, suggesting that IL-6 is a key cytokine in the pathogenesis. In this study, the requirement for IL-6 in endotoxin induced uveitis (EIU) was examined using IL-6 deficient mice. In addition, the capability of murine IL-6 to induce uveitis was tested. Methods: Endotoxin (250 ng) was injected bilaterally into the posterior segment of the eyes in IL-6 deficient and wild type mice (n=8). After 24 hours, eyes were processed for histology and inflammation was determined by scoring the number of infiltrating cells in anterior chamber and posterior segment. To test the ability of IL-6 to induce uveitis, various concentrations of murine IL-6 (1, 4, 10, 20, 200 ng per eye; n=4 per dose) were injected in the posterior segment. Results: Histological examination of the IL-6 deficient and wild-type mice showed no significant difference (p=0.07) in the number of infiltrating cells (63.5±16.9 and 26.5±8.81 respectively). Injection of murine IL-6 in the posterior chamber did not cause significant inflammation at any dose tested. Conclusion: These results show that IL-6, although highly expressed in EIU, is not a key cytokine in the induction of inflammation.
UR - http://www.scopus.com/inward/record.url?scp=26144448107&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=26144448107&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:26144448107
SN - 0146-0404
VL - 37
SP - S543
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
IS - 3
ER -