TY - JOUR
T1 - The role of protein kinase C on amylase secretion from rat parotid gland
AU - Shimomura, Hiromi
AU - Terada, Akane
AU - Hashimoto, Yoshiaki
AU - Soderling, Thomas R.
PY - 1988/2/15
Y1 - 1988/2/15
N2 - The activities of Ca2+·phospholipid-dependent protein kinase(protein kinase C) in rat salivary gland were assayed using synthetic peptide syntide-2(Pro-Leu-Ala-Arg-Thr-Leu-Ser-Val-Ala-Gly-Leu-Pro-Gly-Lys-Lys) as substrate. Levels of the protein kinase C were less than 0.05units/g in the parotid and submandibular glands. The protein kinase C inhibitor, H-7, inhibited amylase secretion from rat parotid gland stimulated by PMA or the combination of phosphatidylserine and 1,2-diolein. The results supported the hypothesis of the secretory mechanism that protein kinase C mediates amylase secretion in rat parotid glands.
AB - The activities of Ca2+·phospholipid-dependent protein kinase(protein kinase C) in rat salivary gland were assayed using synthetic peptide syntide-2(Pro-Leu-Ala-Arg-Thr-Leu-Ser-Val-Ala-Gly-Leu-Pro-Gly-Lys-Lys) as substrate. Levels of the protein kinase C were less than 0.05units/g in the parotid and submandibular glands. The protein kinase C inhibitor, H-7, inhibited amylase secretion from rat parotid gland stimulated by PMA or the combination of phosphatidylserine and 1,2-diolein. The results supported the hypothesis of the secretory mechanism that protein kinase C mediates amylase secretion in rat parotid glands.
UR - http://www.scopus.com/inward/record.url?scp=0024286988&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024286988&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(88)90772-3
DO - 10.1016/0006-291X(88)90772-3
M3 - Article
C2 - 2449208
AN - SCOPUS:0024286988
SN - 0006-291X
VL - 150
SP - 1309
EP - 1314
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -