Abstract
Fluorescence in situ hybridization has become a major tool for analysis of gene and chromosome copy number in normal and malignant tissue. The technique has been applied widely to fresh tissue and dispersed formalin-fixed, paraffin-embedded archival tissue, but its use on sections of archival tissue has largely been limited to sections <6 μ thick. This does not provide intact, uncut nuclei for accurate analysis of gene or chromosome copy number. We report here a method of hybridization to sections >20 μ thick that overcomes these difficulties. Key developments were the use of DNA probes directly labeled with fluorochromes and optical sectioning using laser- scanning confocal microscopy.
Original language | English (US) |
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Pages (from-to) | 237-243 |
Number of pages | 7 |
Journal | American Journal of Pathology |
Volume | 144 |
Issue number | 2 |
State | Published - Feb 1994 |
Externally published | Yes |
ASJC Scopus subject areas
- Pathology and Forensic Medicine