Three-dimensional culture of cells and matrix biomolecules for engineered tissue development and Biokinetics Model validation

Shelley S. Mason, Sean S. Kohles, Randy D. Zelick, Shelley R. Winn, Asit K. Saha

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


There has been considerable progress in cellular and molecular engineering due to recent advances in multiscale technology. Such technologies allow controlled manipulation of physiochemical interactions among cells in tissue culture. In particular, a novel chemomechanical bioreactor has recently been designed for the study of bone and cartilage tissue development, with particular focus on extracellular matrix formation. The bioreactor is equally significant as a tool for validation of mathematical models that explore biokinetic regulatory thresholds (Saha, A. K., and Kohles, S. S., 2010, "A Distinct Catabolic to Anabolic Threshold Due to Single-Cell Nanomechanical Stimulation in a Cartilage Biokinetics Model," J. Nanotechnol. Eng. Med., 1(3), p. 031005; 2010, "Periodic Nanomechanical Stimulation in a Biokinetics Model Identifying Anabolic and Catabolic Pathways Associated With Cartilage Matrix Homeostasis," J. Nanotechnol. Eng. Med., 1(4), p. 041001). In the current study, three-dimensional culture protocols are described for maintaining the cellular and biomolecular constituents within defined parameters. Preliminary validation of the bioreactor's form and function, expected bioassays of the resulting matrix components, and application to biokinetic models are described. This approach provides a framework for future detailed explorations combining multiscale experimental and mathematical analyses, at nanoscale sensitivity, to describe cell and biomolecule dynamics in different environmental regimes.

Original languageEnglish (US)
JournalJournal of Nanotechnology in Engineering and Medicine
Issue number2
StatePublished - May 1 2011
Externally publishedYes


  • Biomechanical signaling
  • Bioreactor
  • Extracellular matrix
  • Nanomedicine

ASJC Scopus subject areas

  • General Materials Science
  • Electrical and Electronic Engineering


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