Abstract
Assessing tumor margin status during surgery is critical to ensure complete resection of cancer tissue; however, current approaches are ineffective and often result in repeat surgery. We present an optical imaging approach for margin assessment using topical application of two fluorescent stains, one targeted to a tumor biomarker and the other a nontargeted reference, to freshly excised specimens. Computing a normalized difference image from fluorescence images of the targeted and untargeted stains suppresses the confounding effects of nonspecific uptake. Applying this approach in excised breast tumor models produced promising tumor-to-normal tissue contrasts that were significantly higher than single-targeted-stain imaging.
Original language | English (US) |
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Pages (from-to) | 5184-5187 |
Number of pages | 4 |
Journal | Optics Letters |
Volume | 38 |
Issue number | 23 |
DOIs | |
State | Published - Dec 1 2013 |
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics