Transposon Tn5 mutagenesis of Brucella abortus

L. D. Smith, F. Heffron

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


We demonstrate that transposon-mediated mutagenesis can be used to construct mutations in the pathogen Brucella abortus. We have used both a plasmid and a bacteriophage to introduce either Tn5 or Tn5 lac into the Brucella chromosome. B. abortus is naturally sensitive to kanamycin. We have selected 22 independent kanamycin-resistant colonies in strain US-19 and 19 colonies in strain 2308. We have demonstrated by Southern hybridization that Tn5 was inserted into the chromosome.

Original languageEnglish (US)
Pages (from-to)2774-2776
Number of pages3
JournalInfection and Immunity
Issue number11
StatePublished - 1987
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases


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