Abstract
We demonstrate that transposon-mediated mutagenesis can be used to construct mutations in the pathogen Brucella abortus. We have used both a plasmid and a bacteriophage to introduce either Tn5 or Tn5 lac into the Brucella chromosome. B. abortus is naturally sensitive to kanamycin. We have selected 22 independent kanamycin-resistant colonies in strain US-19 and 19 colonies in strain 2308. We have demonstrated by Southern hybridization that Tn5 was inserted into the chromosome.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2774-2776 |
| Number of pages | 3 |
| Journal | Infection and Immunity |
| Volume | 55 |
| Issue number | 11 |
| State | Published - 1987 |
| Externally published | Yes |
ASJC Scopus subject areas
- Parasitology
- Microbiology
- Immunology
- Infectious Diseases