Two replication initiation sites on R-plasmid DNA

Jorge H. Crosa; Linda K. Luttropp; Fred Heffron; Stanley Falkow

doi:10.1007/BF00268987

Two replication initiation sites on R-plasmid DNA

Jorge H. Crosa, Linda K. Luttropp, Fred Heffron, Stanley Falkow

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Replicating DNA molecules of a deletion mutant of the conjugative R-plasmid R 6 K are cleaved at a single site by the EcoRI restriction endonuclease. Electron microscope examination and measurements of the EcoRI treated replicative intermediate molecules indicate that replication can be initiated at two sites on the plasmid DNA molecule. The two sites are located at about 23 and 39% of total length, respectively, from the EcoRI cleavage site. About 5% of the replicating molecules use both replication initiation sites simultaneously.

Original language	English (US)
Pages (from-to)	39-50
Number of pages	12
Journal	MGG Molecular & General Genetics
Volume	140
Issue number	1
DOIs	https://doi.org/10.1007/BF00268987
State	Published - Mar 1975
Externally published	Yes

ASJC Scopus subject areas

Genetics

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10.1007/BF00268987

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abstract = "Replicating DNA molecules of a deletion mutant of the conjugative R-plasmid R 6 K are cleaved at a single site by the EcoRI restriction endonuclease. Electron microscope examination and measurements of the EcoRI treated replicative intermediate molecules indicate that replication can be initiated at two sites on the plasmid DNA molecule. The two sites are located at about 23 and 39% of total length, respectively, from the EcoRI cleavage site. About 5% of the replicating molecules use both replication initiation sites simultaneously.",

author = "Crosa, {Jorge H.} and Luttropp, {Linda K.} and Fred Heffron and Stanley Falkow",

year = "1975",

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AU - Crosa, Jorge H.

AU - Luttropp, Linda K.

AU - Heffron, Fred

AU - Falkow, Stanley

PY - 1975/3

Y1 - 1975/3

N2 - Replicating DNA molecules of a deletion mutant of the conjugative R-plasmid R 6 K are cleaved at a single site by the EcoRI restriction endonuclease. Electron microscope examination and measurements of the EcoRI treated replicative intermediate molecules indicate that replication can be initiated at two sites on the plasmid DNA molecule. The two sites are located at about 23 and 39% of total length, respectively, from the EcoRI cleavage site. About 5% of the replicating molecules use both replication initiation sites simultaneously.

AB - Replicating DNA molecules of a deletion mutant of the conjugative R-plasmid R 6 K are cleaved at a single site by the EcoRI restriction endonuclease. Electron microscope examination and measurements of the EcoRI treated replicative intermediate molecules indicate that replication can be initiated at two sites on the plasmid DNA molecule. The two sites are located at about 23 and 39% of total length, respectively, from the EcoRI cleavage site. About 5% of the replicating molecules use both replication initiation sites simultaneously.

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Two replication initiation sites on R-plasmid DNA

Abstract

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