Underperforming light curing procedures trigger detrimental irradiance-dependent biofilm response on incrementally placed dental composites

Haifa Maktabi; Maria Ibrahim; Qoot Alkhubaizi; Michael Weir; Hockin Xu; Howard Strassler; Ana Paula P. Fugolin; Carmem S. Pfeifer; Mary Anne S. Melo

doi:10.1016/j.jdent.2019.04.003

Underperforming light curing procedures trigger detrimental irradiance-dependent biofilm response on incrementally placed dental composites

Haifa Maktabi, Maria Ibrahim, Qoot Alkhubaizi, Michael Weir, Hockin Xu, Howard Strassler, Ana Paula P. Fugolin, Carmem S. Pfeifer, Mary Anne S. Melo

School of Dentistry

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Objectives: Insufficient radiant exposure (J/cm²) may provide an early trigger in a cascade of detrimental responses on incrementally-place composite, especially the bottom layer. This study aimed to assess the influence of poor radiant exposure, the degree of conversion (%DC), water sorption/ solubility and S. mutans biofilm formation on conventional, incrementally placed composites and to establish a relationship between these factors. Methods: Two light units operating at 600 and 1000 mW/cm² and four most common operator-dependent curing conditions had the radiant exposure (R_E) recorded. All the specimens were subjected to S. mutans biofilm model for 14 days. The %DC, biofilm formation expressed by colony-forming units (CFU), water sorption/ solubility and surface roughness/ SEM were assessed. Data were submitted to two-way ANOVA and Tukey post-hoc test (α = 0.05). Pearson correlation was also determined. Results: The influence of R_E on S. mutans CFU values and DC are dependent on the curing conditions and irradiance (p < 0.05). A negative relationship was observed between R_E and biofilm formation. The operator-dependent curing conditions have shown R_E reduction varying from 49.4% to 73.5% in relation to control. The difference in DC between top/bottom of cylinder varied from 13% to 21% for 1000 mW/cm²and from 29% to 53% for LCU600. The roughness, solubility and salivary sorption were greater for low R_E. Conclusion: Poor, deficient curing procedures provide an early trigger in a negative pathway of events for incrementally-place dental composite including a biological response by increased biofilm formation by S. mutans, a relevant factor for secondary caries development. Significance: The susceptibility to variation in the outcomes was R_E -dependent. The optimization of the curing procedures ensures the maximum performance in the chain of events involved in the light curing process of resin-based materials and potentially reduce the risk factors of secondary caries development.

Original language	English (US)
Article number	103110
Journal	Journal of Dentistry
Volume	88
DOIs	https://doi.org/10.1016/j.jdent.2019.04.003
State	Published - Sep 2019

Keywords

Light curing
Oral biofilm
Resin composite
S. mutans
The degree of conversion

ASJC Scopus subject areas

Dentistry(all)

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10.1016/j.jdent.2019.04.003

Cite this

@article{e5a677e7fc1f4c1ab6292af956856a6f,

title = "Underperforming light curing procedures trigger detrimental irradiance-dependent biofilm response on incrementally placed dental composites",

abstract = "Objectives: Insufficient radiant exposure (J/cm2) may provide an early trigger in a cascade of detrimental responses on incrementally-place composite, especially the bottom layer. This study aimed to assess the influence of poor radiant exposure, the degree of conversion (%DC), water sorption/ solubility and S. mutans biofilm formation on conventional, incrementally placed composites and to establish a relationship between these factors. Methods: Two light units operating at 600 and 1000 mW/cm2 and four most common operator-dependent curing conditions had the radiant exposure (RE) recorded. All the specimens were subjected to S. mutans biofilm model for 14 days. The %DC, biofilm formation expressed by colony-forming units (CFU), water sorption/ solubility and surface roughness/ SEM were assessed. Data were submitted to two-way ANOVA and Tukey post-hoc test (α = 0.05). Pearson correlation was also determined. Results: The influence of RE on S. mutans CFU values and DC are dependent on the curing conditions and irradiance (p < 0.05). A negative relationship was observed between RE and biofilm formation. The operator-dependent curing conditions have shown RE reduction varying from 49.4% to 73.5% in relation to control. The difference in DC between top/bottom of cylinder varied from 13% to 21% for 1000 mW/cm2and from 29% to 53% for LCU600. The roughness, solubility and salivary sorption were greater for low RE. Conclusion: Poor, deficient curing procedures provide an early trigger in a negative pathway of events for incrementally-place dental composite including a biological response by increased biofilm formation by S. mutans, a relevant factor for secondary caries development. Significance: The susceptibility to variation in the outcomes was RE -dependent. The optimization of the curing procedures ensures the maximum performance in the chain of events involved in the light curing process of resin-based materials and potentially reduce the risk factors of secondary caries development.",

keywords = "Light curing, Oral biofilm, Resin composite, S. mutans, The degree of conversion",

author = "Haifa Maktabi and Maria Ibrahim and Qoot Alkhubaizi and Michael Weir and Hockin Xu and Howard Strassler and Fugolin, {Ana Paula P.} and Pfeifer, {Carmem S.} and Melo, {Mary Anne S.}",

note = "Funding Information: This study was supported by a seed grant from the University of Maryland School of Dentistry (MM). We gratefully acknowledge the staff of the HS/HSL Innovation Space (University of Maryland Baltimore) for their support in helping us with the design and printing templates and the Biomaterial and Tissue Engineering Division for the use of its facilities. Funding Information: This study was supported by a seed grant from the University of Maryland School of Dentistry (MM) . We gratefully acknowledge the staff of the HS/HSL Innovation Space (University of Maryland Baltimore) for their support in helping us with the design and printing templates and the Biomaterial and Tissue Engineering Division for the use of its facilities. Publisher Copyright: {\textcopyright} 2019",

year = "2019",

month = sep,

doi = "10.1016/j.jdent.2019.04.003",

language = "English (US)",

volume = "88",

journal = "Journal of Dentistry",

issn = "0300-5712",

publisher = "Elsevier BV",

}

TY - JOUR

T1 - Underperforming light curing procedures trigger detrimental irradiance-dependent biofilm response on incrementally placed dental composites

AU - Maktabi, Haifa

AU - Ibrahim, Maria

AU - Alkhubaizi, Qoot

AU - Weir, Michael

AU - Xu, Hockin

AU - Strassler, Howard

AU - Fugolin, Ana Paula P.

AU - Pfeifer, Carmem S.

AU - Melo, Mary Anne S.

N1 - Funding Information: This study was supported by a seed grant from the University of Maryland School of Dentistry (MM). We gratefully acknowledge the staff of the HS/HSL Innovation Space (University of Maryland Baltimore) for their support in helping us with the design and printing templates and the Biomaterial and Tissue Engineering Division for the use of its facilities. Funding Information: This study was supported by a seed grant from the University of Maryland School of Dentistry (MM) . We gratefully acknowledge the staff of the HS/HSL Innovation Space (University of Maryland Baltimore) for their support in helping us with the design and printing templates and the Biomaterial and Tissue Engineering Division for the use of its facilities. Publisher Copyright: © 2019

PY - 2019/9

Y1 - 2019/9

N2 - Objectives: Insufficient radiant exposure (J/cm2) may provide an early trigger in a cascade of detrimental responses on incrementally-place composite, especially the bottom layer. This study aimed to assess the influence of poor radiant exposure, the degree of conversion (%DC), water sorption/ solubility and S. mutans biofilm formation on conventional, incrementally placed composites and to establish a relationship between these factors. Methods: Two light units operating at 600 and 1000 mW/cm2 and four most common operator-dependent curing conditions had the radiant exposure (RE) recorded. All the specimens were subjected to S. mutans biofilm model for 14 days. The %DC, biofilm formation expressed by colony-forming units (CFU), water sorption/ solubility and surface roughness/ SEM were assessed. Data were submitted to two-way ANOVA and Tukey post-hoc test (α = 0.05). Pearson correlation was also determined. Results: The influence of RE on S. mutans CFU values and DC are dependent on the curing conditions and irradiance (p < 0.05). A negative relationship was observed between RE and biofilm formation. The operator-dependent curing conditions have shown RE reduction varying from 49.4% to 73.5% in relation to control. The difference in DC between top/bottom of cylinder varied from 13% to 21% for 1000 mW/cm2and from 29% to 53% for LCU600. The roughness, solubility and salivary sorption were greater for low RE. Conclusion: Poor, deficient curing procedures provide an early trigger in a negative pathway of events for incrementally-place dental composite including a biological response by increased biofilm formation by S. mutans, a relevant factor for secondary caries development. Significance: The susceptibility to variation in the outcomes was RE -dependent. The optimization of the curing procedures ensures the maximum performance in the chain of events involved in the light curing process of resin-based materials and potentially reduce the risk factors of secondary caries development.

AB - Objectives: Insufficient radiant exposure (J/cm2) may provide an early trigger in a cascade of detrimental responses on incrementally-place composite, especially the bottom layer. This study aimed to assess the influence of poor radiant exposure, the degree of conversion (%DC), water sorption/ solubility and S. mutans biofilm formation on conventional, incrementally placed composites and to establish a relationship between these factors. Methods: Two light units operating at 600 and 1000 mW/cm2 and four most common operator-dependent curing conditions had the radiant exposure (RE) recorded. All the specimens were subjected to S. mutans biofilm model for 14 days. The %DC, biofilm formation expressed by colony-forming units (CFU), water sorption/ solubility and surface roughness/ SEM were assessed. Data were submitted to two-way ANOVA and Tukey post-hoc test (α = 0.05). Pearson correlation was also determined. Results: The influence of RE on S. mutans CFU values and DC are dependent on the curing conditions and irradiance (p < 0.05). A negative relationship was observed between RE and biofilm formation. The operator-dependent curing conditions have shown RE reduction varying from 49.4% to 73.5% in relation to control. The difference in DC between top/bottom of cylinder varied from 13% to 21% for 1000 mW/cm2and from 29% to 53% for LCU600. The roughness, solubility and salivary sorption were greater for low RE. Conclusion: Poor, deficient curing procedures provide an early trigger in a negative pathway of events for incrementally-place dental composite including a biological response by increased biofilm formation by S. mutans, a relevant factor for secondary caries development. Significance: The susceptibility to variation in the outcomes was RE -dependent. The optimization of the curing procedures ensures the maximum performance in the chain of events involved in the light curing process of resin-based materials and potentially reduce the risk factors of secondary caries development.

KW - Light curing

KW - Oral biofilm

KW - Resin composite

KW - S. mutans

KW - The degree of conversion

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DO - 10.1016/j.jdent.2019.04.003

M3 - Article

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AN - SCOPUS:85067946534

SN - 0300-5712

VL - 88

JO - Journal of Dentistry

JF - Journal of Dentistry

M1 - 103110

ER -

Underperforming light curing procedures trigger detrimental irradiance-dependent biofilm response on incrementally placed dental composites

Abstract

Keywords

ASJC Scopus subject areas

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