@inproceedings{091d6f03fd904356a84b0f70d66363ad,
title = "Use of Freshly Amputated Human Limbs for Pre-Clinical Evaluation of Molecular-Targeted Fluorescent Probes",
abstract = "We have co-developed a first-in-kind model of fluorophore testing in freshly amputated human limbs. Ex vivo human tissue provides a unique opportunity for the testing of pre-clinical fluorescent agents, collection of imaging data, and histopathologic examination in human tissue prior to performing in vivo experiments. Existing pre-clinical fluorescent agent studies rely primarily on animal models, which do not directly predict fluorophore performance in humans and can result in wasted resources and time if an agent proves ineffective in early human trials. Because fluorophores have no desired therapeutic effect, their clinical utility is based solely on their safety and ability to highlight tissues of interest. Advancing to human trials even via the FDA's phase 0/microdose pathway still requires substantial resources, single-species pharmacokinetic testing, and toxicity testing. In a recently concluded study using amputated human lower limbs, we were able to test successfully a nerve-specific fluorophore in pre-clinical development. This study used systemic administration via vascular cannulization and a cardiac perfusion pump. We envision that this model may assist with early lead agent testing selection for fluorophores with various targets and mechanisms.",
keywords = "Fluorescence imaging, ex vivo imaging, fluorophore testing, human testing, lead agent selection, nerve-specific fluorophore",
author = "Bateman, {Logan M.} and Hebert, {Kendra A.} and Streeter, {Samuel S.} and Nunziata, {Jenna A.} and Barth, {Connor W.} and Wang, {Lei G.} and Gibbs, {Summer L.} and Henderson, {Eric R.}",
note = "Publisher Copyright: {\textcopyright} 2023 SPIE.; Molecular-Guided Surgery: Molecules, Devices, and Applications IX 2023 ; Conference date: 28-01-2023 Through 29-01-2023",
year = "2023",
doi = "10.1117/12.2650356",
language = "English (US)",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Sylvain Gioux and Gibbs, {Summer L.} and Pogue, {Brian W.}",
booktitle = "Molecular-Guided Surgery",
}