Utility of a combination of RD1 and RD2 antigens as a diagnostic marker for tuberculosis

Mamta Kalra; Gopal Krishan Khuller; Ajay Grover; Digambar Behera; Ajay Wanchu; Indu Verma

doi:10.1016/j.diagmicrobio.2009.09.005

Utility of a combination of RD1 and RD2 antigens as a diagnostic marker for tuberculosis

Mamta Kalra, Gopal Krishan Khuller, Ajay Grover, Digambar Behera, Ajay Wanchu, Indu Verma

Arthritis and Rheumatic Diseases

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

We evaluated the diagnostic potential of a cocktail of 4 antigens encoded by regions of difference (RD) 1 and 2 of Mycobacterium tuberculosis, that is, early secretory antigenic target-6, culture filtrate protein-10 (CFP-10), CFP-21, and mycobacterial protein from species tuberculosis-64 (MPT-64) on the basis of antigen and antibody detection by enzyme-linked immunosorbent assay. Parallel detection of antigens and antibodies in the serum samples of pulmonary tuberculosis (PTB) patients resulted in higher sensitivity as compared to either of the single tests in both smear-positive (90%) and smear-negative (60%) PTB patients. In addition, combined detection of antigens and antibodies in the fluids of extrapulmonary tuberculosis (EPTB) patients could detect >90% of the patients with high specificity. These results demonstrate the ability of the combination of antigen and antibody detection assays based on the cocktail of RD antigens to diagnose a substantial number of PTB and EPTB cases with high specificity.

Original language	English (US)
Pages (from-to)	153-161
Number of pages	9
Journal	Diagnostic Microbiology and Infectious Disease
Volume	66
Issue number	2
DOIs	https://doi.org/10.1016/j.diagmicrobio.2009.09.005
State	Published - Feb 2010

Keywords

Antibody detection
Antigen detection
ELISA
RD antigens
Tuberculosis

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

Access to Document

10.1016/j.diagmicrobio.2009.09.005

Cite this

@article{cd96676ecd93443a8220c25f85228f8f,

title = "Utility of a combination of RD1 and RD2 antigens as a diagnostic marker for tuberculosis",

abstract = "We evaluated the diagnostic potential of a cocktail of 4 antigens encoded by regions of difference (RD) 1 and 2 of Mycobacterium tuberculosis, that is, early secretory antigenic target-6, culture filtrate protein-10 (CFP-10), CFP-21, and mycobacterial protein from species tuberculosis-64 (MPT-64) on the basis of antigen and antibody detection by enzyme-linked immunosorbent assay. Parallel detection of antigens and antibodies in the serum samples of pulmonary tuberculosis (PTB) patients resulted in higher sensitivity as compared to either of the single tests in both smear-positive (90%) and smear-negative (60%) PTB patients. In addition, combined detection of antigens and antibodies in the fluids of extrapulmonary tuberculosis (EPTB) patients could detect >90% of the patients with high specificity. These results demonstrate the ability of the combination of antigen and antibody detection assays based on the cocktail of RD antigens to diagnose a substantial number of PTB and EPTB cases with high specificity.",

keywords = "Antibody detection, Antigen detection, ELISA, RD antigens, Tuberculosis",

author = "Mamta Kalra and Khuller, {Gopal Krishan} and Ajay Grover and Digambar Behera and Ajay Wanchu and Indu Verma",

note = "Funding Information: Recombinant ESAT-6 and MPT-64 were kindly provided by Dr Karen Dobos as part of NIH, NIAID contract no. HHSN266200400091C, entitled “Tuberculosis vaccine testing and research materials”, which was awarded to the Colorado State University. The authors thank Dr Suraj B Sable, CDC, Atlanta, GA, and Mr Javaid Ahmad of Postgraduate Medical Education and Research (PGIMER), Chandigarh, India, for helpful discussions and critical reading of the manuscript. The help from Dr Satyavati Rana, Professor, Department of Gastroenterology, PGIMER, as well as the staff at Revised National Tuberculosis Control Program Center, PGIMER, Chandigarh, and residents at TB and Chest Diseases Hospital, Patiala, India, in collecting patient samples is gratefully acknowledged.",

year = "2010",

month = feb,

doi = "10.1016/j.diagmicrobio.2009.09.005",

language = "English (US)",

volume = "66",

pages = "153--161",

journal = "Diagnostic Microbiology and Infectious Disease",

issn = "0732-8893",

publisher = "Elsevier Inc.",

number = "2",

}

TY - JOUR

T1 - Utility of a combination of RD1 and RD2 antigens as a diagnostic marker for tuberculosis

AU - Kalra, Mamta

AU - Khuller, Gopal Krishan

AU - Grover, Ajay

AU - Behera, Digambar

AU - Wanchu, Ajay

AU - Verma, Indu

N1 - Funding Information: Recombinant ESAT-6 and MPT-64 were kindly provided by Dr Karen Dobos as part of NIH, NIAID contract no. HHSN266200400091C, entitled “Tuberculosis vaccine testing and research materials”, which was awarded to the Colorado State University. The authors thank Dr Suraj B Sable, CDC, Atlanta, GA, and Mr Javaid Ahmad of Postgraduate Medical Education and Research (PGIMER), Chandigarh, India, for helpful discussions and critical reading of the manuscript. The help from Dr Satyavati Rana, Professor, Department of Gastroenterology, PGIMER, as well as the staff at Revised National Tuberculosis Control Program Center, PGIMER, Chandigarh, and residents at TB and Chest Diseases Hospital, Patiala, India, in collecting patient samples is gratefully acknowledged.

PY - 2010/2

Y1 - 2010/2

N2 - We evaluated the diagnostic potential of a cocktail of 4 antigens encoded by regions of difference (RD) 1 and 2 of Mycobacterium tuberculosis, that is, early secretory antigenic target-6, culture filtrate protein-10 (CFP-10), CFP-21, and mycobacterial protein from species tuberculosis-64 (MPT-64) on the basis of antigen and antibody detection by enzyme-linked immunosorbent assay. Parallel detection of antigens and antibodies in the serum samples of pulmonary tuberculosis (PTB) patients resulted in higher sensitivity as compared to either of the single tests in both smear-positive (90%) and smear-negative (60%) PTB patients. In addition, combined detection of antigens and antibodies in the fluids of extrapulmonary tuberculosis (EPTB) patients could detect >90% of the patients with high specificity. These results demonstrate the ability of the combination of antigen and antibody detection assays based on the cocktail of RD antigens to diagnose a substantial number of PTB and EPTB cases with high specificity.

AB - We evaluated the diagnostic potential of a cocktail of 4 antigens encoded by regions of difference (RD) 1 and 2 of Mycobacterium tuberculosis, that is, early secretory antigenic target-6, culture filtrate protein-10 (CFP-10), CFP-21, and mycobacterial protein from species tuberculosis-64 (MPT-64) on the basis of antigen and antibody detection by enzyme-linked immunosorbent assay. Parallel detection of antigens and antibodies in the serum samples of pulmonary tuberculosis (PTB) patients resulted in higher sensitivity as compared to either of the single tests in both smear-positive (90%) and smear-negative (60%) PTB patients. In addition, combined detection of antigens and antibodies in the fluids of extrapulmonary tuberculosis (EPTB) patients could detect >90% of the patients with high specificity. These results demonstrate the ability of the combination of antigen and antibody detection assays based on the cocktail of RD antigens to diagnose a substantial number of PTB and EPTB cases with high specificity.

KW - Antibody detection

KW - Antigen detection

KW - ELISA

KW - RD antigens

KW - Tuberculosis

UR - http://www.scopus.com/inward/record.url?scp=73749085775&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=73749085775&partnerID=8YFLogxK

U2 - 10.1016/j.diagmicrobio.2009.09.005

DO - 10.1016/j.diagmicrobio.2009.09.005

M3 - Article

C2 - 19833469

AN - SCOPUS:73749085775

SN - 0732-8893

VL - 66

SP - 153

EP - 161

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 2

ER -

Utility of a combination of RD1 and RD2 antigens as a diagnostic marker for tuberculosis

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this