Abstract
There is great interest lately in the availability of analytical methods for quantification of 3-iodothyronamine from blood and tissues. To date, no validated method for determination of 3-iodothyronamine from biological matrices has been described. Detailed in this report is an LC-MS/MS method that permits accurate and reproducible quantification of pharmacological concentrations of 3-iodothyronamine from rat serum, with a 0.0008 M lower limit of quantification. Endogenous 3-iodothyronamine was observed from rodent and human serum (0.2 mL) at the method limit of detection. In summary, the LC-MS/MS method enables quantification of circulating 3-iodothyronamine to allow examination of a relationship with biological activity.
Original language | English (US) |
---|---|
Pages (from-to) | 55-59 |
Number of pages | 5 |
Journal | Journal of Chromatography A |
Volume | 1210 |
Issue number | 1 |
DOIs | |
State | Published - Nov 7 2008 |
Keywords
- 3-Iodothyronamine
- LC-MS/MS
- Serum
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Organic Chemistry