Vasopressin receptors in the brain, liver and kidney of rats following osmotic stimulation

Rainer Landgraf; Patricia Szot; Daniel M. Dorsa

doi:10.1016/0006-8993(91)90066-5

Vasopressin receptors in the brain, liver and kidney of rats following osmotic stimulation

Rainer Landgraf, Patricia Szot, Daniel M. Dorsa

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The binding site concentration (B_max) and equilibrium dissociation constant (K_d) for [³H]-arginine vasopressin (AVP) binding sites were measured in limbic brain areas (septum, dorsal hippocampus, amygdala) and liver and kidney of control and osmotically stimulated male Wistar rats. Membrane binding was performed in these five areas 30, 60 and 180 min following intraperitoneal injection of hypertonic saline. This paradigm resulted in no significant change in binding characteristics in the septum, dorsal hippocampus, amygdala and liver from control treated rats. In contrast, the kidney B_max was significantly reduced 60min following osmotic stimulation, with no effect on affinity. These results also suggest that AVP receptors in the CNS are relatively resistant to regulatory effects of an acute AVP exposure.

Original language	English (US)
Pages (from-to)	287-290
Number of pages	4
Journal	Brain research
Volume	544
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(91)90066-5
State	Published - Mar 29 1991
Externally published	Yes

Keywords

Kidney
Osmotic stimulation
Receptor
Vasopressin

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(91)90066-5

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abstract = "The binding site concentration (Bmax) and equilibrium dissociation constant (Kd) for [3H]-arginine vasopressin (AVP) binding sites were measured in limbic brain areas (septum, dorsal hippocampus, amygdala) and liver and kidney of control and osmotically stimulated male Wistar rats. Membrane binding was performed in these five areas 30, 60 and 180 min following intraperitoneal injection of hypertonic saline. This paradigm resulted in no significant change in binding characteristics in the septum, dorsal hippocampus, amygdala and liver from control treated rats. In contrast, the kidney Bmax was significantly reduced 60min following osmotic stimulation, with no effect on affinity. These results also suggest that AVP receptors in the CNS are relatively resistant to regulatory effects of an acute AVP exposure.",

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AU - Landgraf, Rainer

AU - Szot, Patricia

AU - Dorsa, Daniel M.

PY - 1991/3/29

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N2 - The binding site concentration (Bmax) and equilibrium dissociation constant (Kd) for [3H]-arginine vasopressin (AVP) binding sites were measured in limbic brain areas (septum, dorsal hippocampus, amygdala) and liver and kidney of control and osmotically stimulated male Wistar rats. Membrane binding was performed in these five areas 30, 60 and 180 min following intraperitoneal injection of hypertonic saline. This paradigm resulted in no significant change in binding characteristics in the septum, dorsal hippocampus, amygdala and liver from control treated rats. In contrast, the kidney Bmax was significantly reduced 60min following osmotic stimulation, with no effect on affinity. These results also suggest that AVP receptors in the CNS are relatively resistant to regulatory effects of an acute AVP exposure.

AB - The binding site concentration (Bmax) and equilibrium dissociation constant (Kd) for [3H]-arginine vasopressin (AVP) binding sites were measured in limbic brain areas (septum, dorsal hippocampus, amygdala) and liver and kidney of control and osmotically stimulated male Wistar rats. Membrane binding was performed in these five areas 30, 60 and 180 min following intraperitoneal injection of hypertonic saline. This paradigm resulted in no significant change in binding characteristics in the septum, dorsal hippocampus, amygdala and liver from control treated rats. In contrast, the kidney Bmax was significantly reduced 60min following osmotic stimulation, with no effect on affinity. These results also suggest that AVP receptors in the CNS are relatively resistant to regulatory effects of an acute AVP exposure.

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KW - Osmotic stimulation

KW - Receptor

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Vasopressin receptors in the brain, liver and kidney of rats following osmotic stimulation

Abstract

Keywords

ASJC Scopus subject areas

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